M W Roomi, N W Roomi, B Bhanap, A Niedzwiecki, M Rath;
Cancer and Clinical Oncology 2012; Vol. 1, No. 2,
primary bone cancers. Surgery is the main treatment option because chondrosarcoma typically does not respond to radiation and chemotherapy. Cancer mortality usually results from the local and distant metastasis. A nutrient mixture containing ascorbic acid, lysine, proline, green tea extract, was tested on chondrosarcoma cells SW-1353 for viability, matrix metalloproteinase expression, Matrigel invasion, morphology and apoptosis.
The SW-1353 cells were grown in appropriate media and treated with the nutrient mixture at 10, 50, 100, 500 and 1000 µg/ml concentrations. Cells were also treated with PMA (100ng/ml) for MMP-9 stimulation. Cell proliferation was carried out by MTT assay, MMPs by zymography, invasion through Matrigel. Morphology and apoptosis were also conducted. The nutrient mixture did not exhibit toxicity at 100 µg/ml, but showed 40% inhibition at 1000 µg/ml. Zymography demonstrated two bands, for MMP-2 and MMP-9. PMA treatment further enhanced MMP-9 expression. The nutrient mixture inhibited expression of both MMPs in a dose dependent manner. Matrigel invasion was reduced by 40%, 70%, 88% and 100% at 50, 100, 500 and 1000 µg/ml respectively. The nutrient mixture induced slight apoptosis at 250 µg/ml, moderate at 500 µg/ml and significant at 1000 µg/ml concentrations. Morphology showed slight changes at the highest concentrations. The nutrient mixture significantly inhibited all the important hallmarks for cancer progression, suggesting that it has a possibility for exploration as a significant therapeutic entity in chondrosarcoma.
Key words: chondrosarcoma, nutrient mixture, MMP, Matrigel invasion, apoptosis