Inhibition of Malignant Mesothelioma Cell Matrix Metalloproteinase Production and Invasion by a Novel Nutrient mixture

We investigated the effect of NM on MM cell line MSTO-211 H proliferation (by MTT assay), MMP activity (by gelatinase zymography), invasion (through Matrigel) and morphology (by H&E staining).

MMP-2 and PMA-induced MMP-9 secretion were inhibited by NM in a dose-dependent fashion with virtual total inhibition at 500 mg/ml NM. Invasion through Matrigel was inhibited at 50, 100 and 500 mg/ml by 27, 36 and 100% respectively. NM was not toxic to the MM cell line, and H&E staining did not indicate any changes even at the highest concentration.

In conclusion, NM significantly inhibited MM cell MMP secretion and invasion- both important parameters for cancer prevention, suggesting NM as an effective treatment strategy for MM.

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