A Nutrient Mixture Inhibits MMP Secretion, Invasion, Growth, and Induction of Apoptosis in Human Tongue Cancer Cell Line SC-255

This study was undertaken to determine if the nutrient mixture is useful in inhibiting various parameters of cancer progression on human tongue cancer cell line SC-255. SC-255 cells were grown in a Dulbecco’s Eagle medium and treated with the nutrient mixture at 0, 10, 50, 100, 500 and 1000 µg/ml, in triplicate. The nutrient mixture exhibited 20% and 30% toxicity at 500 and 1000 µg/ml, respectively. Zymography demonstrated the expression of MMP-2 and MMP-9; and PMA treatment further enhanced MMP-9 activity. The nutrient mixture inhibited the secretion of MMP-2 and MMP-9 in a dose-dependent fashion with a total inhibition at 500 µg/ml. Matrigel invasion was significantly reduced by 40%, 80% and 100% at 100, 500 and 1000 µg/ml, respectively. The nutrient mixture also inhibited cell migration and induced apoptosis in a dose response fashion. Thus, the nutrient mixture may have a potential in the tongue cancer treatment.



Keywords:  Oral Cancer; Nutrient Mixture; MMP; Apoptosis


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M. W Roomi, N W. Roomi, B Bhanap, A Niedzwiecki, M Rath